shinegene blogspot.com
Gene Synthesis
Protocol 1 T-Vector Preparation. You may choose any plasmid which satisfies your needs as long as the plasmid contains a unique blunt-end restriction site in the multiple cloning sites polylinker for example, the EcoR V site of the pBluescript plasmid, which has bluewhite color selection, may be used for cloning. For expression in eukaryotic cells the pcDNA3 plasmid can be used. The T-vectors are prepared in batch and stored as aliquots at -20C for future use. 2 Incubate at 37C for 1-2 h. 1 Add 1 m.
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Gene SynthesisDESCRIPTION
Protocol 1 T-Vector Preparation. You may choose any plasmid which satisfies your needs as long as the plasmid contains a unique blunt-end restriction site in the multiple cloning sites polylinker for example, the EcoR V site of the pBluescript plasmid, which has bluewhite color selection, may be used for cloning. For expression in eukaryotic cells the pcDNA3 plasmid can be used. The T-vectors are prepared in batch and stored as aliquots at -20C for future use. 2 Incubate at 37C for 1-2 h. 1 Add 1 m.CONTENT
This web page shinegene.blogspot.com states the following, "You may choose any plasmid which satisfies your needs as long as the plasmid contains a unique blunt-end restriction site in the multiple cloning sites polylinker for example, the EcoR V site of the pBluescript plasmid, which has bluewhite color selection, may be used for cloning." We saw that the webpage said " For expression in eukaryotic cells the pcDNA3 plasmid can be used." It also said " The T-vectors are prepared in batch and stored as aliquots at -20C for future use. 2 Incubate at 37C for 1-2 h."SEEK SIMILAR DOMAINS
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Shine Gifts Darwin, Cullen Bay and the Waterfront Gifts for everyone
Shine Gifts Darwin, Cullen Bay and the Waterfront. Gift, art and jewellery paradise in Darwin. Shine Cullen Bay 63 Marina Blvd. Shine Waterfront Shop 3E, 19 Kitchener Dr. Welcome to the Shine Gifts online shop. This page is an overview of everything in our online store.
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