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Plasmid Project

Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled - pGLO. Placed in foam tube rack. Scooped one colony of E. Coli from starter plate. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. PGLO LBamp, LBampara. PGLO LB, LBamp. Heat shocked tubes, 0 C ice, 42 C water 50 sec., 0 C ice 2 min. Changed to dryer foam rack. Worried about .

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Plasmid Project

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Tuesday, December 18, 2007. PGLO plasmid cultures, 32C incubation and mixing. GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled - pGLO. Placed in foam tube rack. Scooped one colony of E. Coli from starter plate. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. PGLO LBamp, LBampara. PGLO LB, LBamp. Heat shocked tubes, 0 C ice, 42 C water 50 sec., 0 C ice 2 min. Changed to dryer foam rack. Worried about .

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This web page plasmid-science-fair.blogspot.com states the following, "Tuesday, December 18, 2007." We saw that the webpage said " PGLO plasmid cultures, 32C incubation and mixing." It also said " GFP proteins purified with HIC chromatography. Wednesday, November 28, 2007. Got 2 tubes, labeled - pGLO. Placed in foam tube rack. Scooped one colony of E. Inserted into tubes and spun. Put pGLO plasmids into pGLO tube. Put tubes in ice for 10 min. Heat shocked tubes, 0 C ice, 42 C water 50 sec. , 0 C ice 2 min. Changed to dryer foam rack."

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