lab-monkeys blogspot.com

Desperate Lab Monkeys

Saturday, November 11, 2006. Week 19 - DNA Extraction contd - daphne. DNA Extraction from the lower layer. 1 remove aqueous phase containing RNA COMPLETELY and transfer the organic phase to a clean microcentrifugr tube remove tissue debri. 2 add 0.3 ml of 100 Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10 ethanol.

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LINKS TO WEB SITE

.Guinea 00 work.

Sunday, November 12, 2006. Measures the amount of blood glucose level present in the body at that time of blood collection and is use for the diagnosis and monitoring of treatment of hyperglycemia and hypoglycemia. Finally, the gel is dried at 100oC.

micRobes miT blOod

Saturday, September 23, 2006. Insulin-dependent diabetes since age 17.

Med Tech in Training

Wednesday, November 01, 2006. I have observed both the finger prick and heel prick procedure. Heel pricks are usually done on premature babies. In addition for premature babies, the reticulocyte count test may also be requested.

SIP Experience

Wednesday, October 25, 2006. My lab participates in Biorad Unity interlaboratory program. This interlaboratory program is performed by Biorad. It is done every month where the control values that are run everyday are sent to Biorad where they will compare your lab values with all the other lab participating in that particular program. This program has a lot of advantages and is one of the most powerful tool at the moment. Be one of thousands of lab site participants worldwide, more than any other.

Internship Experiences

Attachment from 26 June to 10 November. Saturday, November 04, 2006. Reagent labeling and hazard signs. 2 All inadequately labeled containers should be discarded. If the contents are unknown, advice should be sought from the chemical waste disposal company. 3 Labels should be attached firmly to the body of a container, not the lid.

Student Internship Program

Monday, January 29, 2007. It brings together the quality management concepts outlined in ISO 9001 and the competency requirements of ISO17025. Specify and approve all methodology used.

BLOGGERS jane, maybelline, valerie, huiyan

Thursday, October 26, 2006. The kit is stored at 2-8 degree celcius and microwells sealed in a dry bag. The test reagents cannot be exposed to heat, sun or strong light during storage or usage. This kit also contain components preserved with sodium azide. Sodium azide may react with lead and copper to form explosive metal azide. On disposal, it must be flushed with a large volume of water. Tuesday, October 24, 2006.

SIP works in progress

Saturday, October 21, 2006. Mounting of Gynaecological Cell Samples. Having been mounting slides for a month, I must say that although the method of cover slipping a slide may seem very easy, it actually requires practice to achieve well-mounted slides, free of air bubbles and artefacts at a reasonable speed. 1 Removal of Visible Air Bubbles.

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Desperate Lab Monkeys

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Saturday, November 11, 2006. Week 19 - DNA Extraction contd - daphne. DNA Extraction from the lower layer. 1 remove aqueous phase containing RNA COMPLETELY and transfer the organic phase to a clean microcentrifugr tube remove tissue debri. 2 add 0.3 ml of 100 Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0.1M of sodium citrate in 10 ethanol.

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This web page lab-monkeys.blogspot.com states the following, "Saturday, November 11, 2006." We saw that the webpage said " Week 19 - DNA Extraction contd - daphne." It also said " DNA Extraction from the lower layer. 1 remove aqueous phase containing RNA COMPLETELY and transfer the organic phase to a clean microcentrifugr tube remove tissue debri. 3 ml of 100 Ethanol and mix by inversion. 3 incubate at room temperature for 3 min, then centrifuge at 2000. For 5 min at 4 degrees C. 4 remove the phenol-ethanol supernatant and wash the DNA pellet with 1ml of 0. 1M of sodium citrate in 10 ethanol."

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