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Academic Pursuit-Approaching the TruthDESCRIPTION
Academic Pursuit-Approaching the Truth. Thursday, January 26, 2006. 分子克隆常用载体http www.bio.davidson.educoursesgenomicsmethodreporters.html. 一 pBR322质粒是4362bp的环状双链DNA载体有2个抗药性基因 四环素和氨苄青霉素 一个复制起始点和多个用于克隆的限制酶切点 二 pUC系列质粒是2.7Kb的双链DNA质粒有一个复制起点一个氨苄青霉素抗性基因和一个多克隆位点多克隆位点处于表达LacZ基因产物-β-半乳糖苷酶的氨基端片段用pUC质粒转化LacZ基因有突变的大肠杆菌株 M15 时因为由质粒表达的α 肽补充了大肠杆菌却失的α 肽所以恢复了分解半乳糖的能力 二 噬粒 phagemid 在质粒DNA中插入一段单链噬菌体的复制起始点DNA即构成了噬粒如pGEM-3Zf - 就是一种噬粒 After overnight incubation, store the plate in 4 0 C for several hours46.CONTENT
This web page ben1011.blogspot.com states the following, "Thursday, January 26, 2006." We saw that the webpage said " 一 pBR322质粒是4362bp的环状双链DNA载体有2个抗药性基因 四环素和氨苄青霉素 一个复制起始点和多个用于克隆的限制酶切点 二 pUC系列质粒是2." It also said "7Kb的双链DNA质粒有一个复制起点一个氨苄青霉素抗性基因和一个多克隆位点多克隆位点处于表达LacZ基因产物-β-半乳糖苷酶的氨基端片段用pUC质粒转化LacZ基因有突变的大肠杆菌株 M15 时因为由质粒表达的α 肽补充了大肠杆菌却失的α 肽所以恢复了分解半乳糖的能力 二 噬粒 phagemid 在质粒DNA中插入一段单链噬菌体的复制起始点DNA即构成了噬粒如pGEM-3Zf - 就是一种噬粒 After overnight incubation, store the plate in 4 0 C for several hours46."